This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II t
Reconstitute with 20mM Tris (pH8.0) and 150mM NaCl (pH8.0) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris (pH8.0), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose, ProClin 300.
Expression System:
HEK293 cells
Formulierung:
Lyophilized powder
Sequenz:
N-terminal His-Tag, Gly57~Leu235 (NP_001265530.1)
Anwendungsbeschreibung:
TNF-a (Tumor necrosis factor), being an endogenous pyrogen, is able to induce fever, necrosis, inflammation and to inhibit tumorigenesis. As reported, TNF-a could inhibit the proliferation and induce necrosis of A549 cells, and the concentration of IL-1beta
GTX00288-pro Functional Assay Image
SDS-PAGE analysis of GTX00288-pro Mouse TNF alpha protein (active).
WB analysis of GTX00288-pro Mouse TNF alpha protein (active).
Cell necrosis effect of GTX00288-pro Mouse TNF alpha protein (active). (A) Unstimulated A549 cells cultured in DMEM for 72hrs. (B) A549 cells cultured in DMEM, stimulated with 10 ng/ml TNF alpha for 72hrs.
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