Mouse TNF alpha protein, His tag (active), Unconjugated

Product Overview

• Article Name: Mouse TNF alpha protein, His tag (active), Unconjugated
• Biozol Catalog Number: GTX00288-PRO
• Supplier Catalog Number: GTX00288-pro
• Alternative Catalog Number: GTX00288-PRO-10
• Manufacturer: GeneTex
• Category: Proteine/Peptide
• Application: FA
• Species Reactivity: Mouse
• Conjugation: Unconjugated
• Alternative Names: tumor necrosis factor , DIF , TNF-a , TNF-alpha , TNFSF2 , TNFalpha , Tnfa , Tnfsf1a , Tnlg1f

Product Description

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II t

Product Properties

• NCBI: 21926
• UniProt: P06804
• Buffer: Reconstitute with 20mM Tris (pH8.0) and 150mM NaCl (pH8.0) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris (pH8.0), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose, ProClin 300.
• Expression System: HEK293 cells
• Form: Lyophilized powder
• Sequence: N-terminal His-Tag, Gly57~Leu235 (NP_001265530.1)

Application Notes

• Application Notes: TNF-a (Tumor necrosis factor), being an endogenous pyrogen, is able to induce fever, necrosis, inflammation and to inhibit tumorigenesis. As reported, TNF-a could inhibit the proliferation and induce necrosis of A549 cells, and the concentration of IL-1beta

Images

GTX00288-pro Functional Assay Image

SDS-PAGE analysis of GTX00288-pro Mouse TNF alpha protein (active).

WB analysis of GTX00288-pro Mouse TNF alpha protein (active).

Cell necrosis effect of GTX00288-pro Mouse TNF alpha protein (active).
(A) Unstimulated A549 cells cultured in DMEM for 72hrs.
(B) A549 cells cultured in DMEM, stimulated with 10 ng/ml TNF alpha for 72hrs.