ZHX2 antibody [C1C3], Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: ZHX2 antibody [C1C3], Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX112232
• Hersteller Artikelnummer: GTX112232
• Alternativnummer: GTX112232-100,GTX112232-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ChIP, ICC, IHC-Fr, IP, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human ZHX2. The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: zinc fingers and homeoboxes 2 , AFR1 , RAF

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.7 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 92
• Sensitivitaet: Knockdown/Knockout validation was supported by references (PMID:36037364)
• NCBI: 22882
• UniProt: Q9Y6X8
• Puffer: 1XPBS (pH7), 20% Glycerol, 0.025% ProClin 300.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX112232 ICC/IF Image

Immunohistochemical analysis of paraffin-embedded SAS Xenograft , using ZHX2(GTX112232) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZHX2(GTX112232) antibody at 1:200 dilution.

Immunoprecipitation of ZHX2 protein from MCF-7 whole cell extracts using 5 μg of ZHX2 antibody [C1C3] (GTX112232).
Western blot analysis was performed using ZHX2 antibody [C1C3] (GTX112232).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZHX2 antibody [C1C3] (GTX112232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.