ZHX2 antibody [C1C3], Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: ZHX2 antibody [C1C3], Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX112232
• Supplier Catalog Number: GTX112232
• Alternative Catalog Number: GTX112232-100,GTX112232-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, ICC, IHC-Fr, IP, WB
• Species Reactivity: Human, Mouse
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human ZHX2. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: zinc fingers and homeoboxes 2 , AFR1 , RAF

Product Properties

• Clonality: Polyclonal
• Concentration: 0.7 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 92
• Sensitivity: Knockdown/Knockout validation was supported by references (PMID:36037364)
• NCBI: 22882
• UniProt: Q9Y6X8
• Buffer: 1XPBS (pH7), 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX112232 ICC/IF Image

Immunohistochemical analysis of paraffin-embedded SAS Xenograft , using ZHX2(GTX112232) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZHX2(GTX112232) antibody at 1:200 dilution.

Immunoprecipitation of ZHX2 protein from MCF-7 whole cell extracts using 5 μg of ZHX2 antibody [C1C3] (GTX112232).
Western blot analysis was performed using ZHX2 antibody [C1C3] (GTX112232).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZHX2 antibody [C1C3] (GTX112232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.