eIF3e antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: eIF3e antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX112342
• Hersteller Artikelnummer: GTX112342
• Alternativnummer: GTX112342-100,GTX112342-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IHC-P, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Recombinant protein encompassing a sequence within the center region of human eIF3e. The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: eukaryotic translation initiation factor 3 subunit E , EIF3-P48 , EIF3S6 , INT6 , eIF3-p46

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 52
• NCBI: 3646
• UniProt: P60228
• Puffer: 1XPBS (pH7), 1% BSA, 20% Glycerol, 0.01% Thimerosal.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX112342 IHC-P Image

Sample (30 ug of whole cell lysate)
A: BCL-1
10% SDS PAGE
GTX112342 diluted at 1:2000

eIF3e antibody detects EIF3E protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: EIF3E protein stained by eIF3e antibody (GTX112342) diluted at 1:500.
Blue: Hoechst 33342 staining.

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
10% SDS PAGE
GTX112342 diluted at 1:2000

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using eIF3e(GTX112342) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min