WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX112342 IHC-P Image
Sample (30 ug of whole cell lysate) A: BCL-1 10% SDS PAGE GTX112342 diluted at 1:2000
eIF3e antibody detects EIF3E protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: EIF3E protein stained by eIF3e antibody (GTX112342) diluted at 1:500. Blue: Hoechst 33342 staining.
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji 10% SDS PAGE GTX112342 diluted at 1:2000
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using eIF3e(GTX112342) antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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