Dengue virus NS4B protein antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Dengue virus NS4B protein antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX124250
• Hersteller Artikelnummer: GTX124250
• Alternativnummer: GTX124250-100,GTX124250-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FACS, ICC, WB
• Spezies Reaktivität: Virus
• Immunogen: Full length Dengue virus 2 NS4B Recombinant protein (Dengue virus 2 (strain 16681 PDK 53))
• Konjugation: Unconjugated
• Alternative Synonym: Dengue Virus Non-structural protein 4B , Dengue Virus NS4B , DENV Non-structural protein 4B , DENV NS4B

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 27
• Sensitivitaet: This antibody was raised against Dengue virus 2 NS4B protein, which may cross react with NS4B protein of Dengue virus 3 but does not recignize with NS4B protein of JEV.
• Puffer: 1XPBS (pH7), 1% BSA, 20% Glycerol, 0.01% Thimerosal.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX124250 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS4B (Dengue virus 2)(GTX124250) antibody at 1:2000 dilution.

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX124250) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
10% SDS PAGE
GTX124250 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
12% SDS-PAGE
NS4B (Dengue virus) antibody (GTX124250) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS4B protein antibody (GTX124250) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.