Dengue virus NS4B protein antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
GTX124250
- Images (6)
| Article Name: | Dengue virus NS4B protein antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | GTX124250 |
| Supplier Catalog Number: | GTX124250 |
| Alternative Catalog Number: | GTX124250-100,GTX124250-25 |
| Manufacturer: | GeneTex |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FACS, ICC, WB |
| Species Reactivity: | Virus |
| Immunogen: | Full length Dengue virus 2 NS4B Recombinant protein (Dengue virus 2 (strain 16681 PDK 53)) |
| Conjugation: | Unconjugated |
| Alternative Names: | Dengue Virus Non-structural protein 4B , Dengue Virus NS4B , DENV Non-structural protein 4B , DENV NS4B |
| Clonality: | Polyclonal |
| Concentration: | 1 mg/ml (Please refer to the vial label for the specific concentration.) |
| Molecular Weight: | 27 |
| Sensitivity: | This antibody was raised against Dengue virus 2 NS4B protein, which may cross react with NS4B protein of Dengue virus 3 but does not recignize with NS4B protein of JEV. |
| Buffer: | 1XPBS (pH7), 1% BSA, 20% Glycerol, 0.01% Thimerosal. |
| Purity: | Purified by antigen-affinity chromatography. |
| Form: | Liquid |
| Application Notes: | WB: 1:500-1:3000. ICC/IF: 1:100-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications. |
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GTX124250 ICC/IF Image |
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Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS4B (Dengue virus 2)(GTX124250) antibody at 1:2000 dilution. |
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NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH. Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX124250) diluted at 1:2000. Blue: Hoechst 33342 staining. |
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Sample (20 μg of whole cell lysate) A: BHK-21 B: Dengue virus 2 infect BHK-21 C: JEV infect BHK-21 10% SDS PAGE GTX124250 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
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NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein by western blot analysis. A. 30 μg BHK-21 whole cell extract B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells 12% SDS-PAGE NS4B (Dengue virus) antibody (GTX124250) dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
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Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS4B protein antibody (GTX124250) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
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