SARS-CoV-2 (COVID-19) Spike overexpression 293T whole cell lysate

Artikelnummer: GTX535664
Artikelname: SARS-CoV-2 (COVID-19) Spike overexpression 293T whole cell lysate
Artikelnummer: GTX535664
Hersteller Artikelnummer: GTX535664
Alternativnummer: GTX535664-200
Hersteller: GeneTex
Kategorie: Proteine/Peptide
Applikation: WB, ELISA
Spezies Reaktivität: SARS Coronavirus 2
Alternative Synonym: CORONA, COVID, COVID-19 Spike, NCOV, SARS-COV-2, COVID19, COVID-19, SARS-CoV-2 Spike, COV-2, COV2, 2019-nCoV Spike, SARS-COV
Konzentration: 1 mg/ml
Puffer: 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.5% sodium deoxycholate, 1% Triton X-100, 0.25% SDS, 1 mM PMSF, 5 µg/ml Leupeptin, 2 µg/ml Aprotinin, 1 µg/ml Pepstatin A, 0.025% ProClin 300
SARS-CoV-2 (COVID-19) spike 293T overexpression cell lysates (GTX535664) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Non-transfected and transfected 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike antibody (GTX135356) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.