beta Tubulin 3/ Tuj1 antibody [GT886], IgG1, Clone: Monoclonal, Unconjugated, Human

Artikelnummer: GTX631830
Artikelname: beta Tubulin 3/ Tuj1 antibody [GT886], IgG1, Clone: Monoclonal, Unconjugated, Human
Artikelnummer: GTX631830
Hersteller Artikelnummer: GTX631830
Alternativnummer: GTX631830-100,GTX631830-25
Hersteller: GeneTex
Wirt: Human
Kategorie: Antikörper
Applikation: IHC-Fr, ICC, WB, IHC-P, IP
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Tubulin 3/ Tuj1. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4
Klonalität: Monoclonal
Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: Monoclonal
Molekulargewicht: 50
Isotyp: IgG1
NCBI: 10381
UniProt: Q13509
Puffer: PBS
Reinheit: Purified by antigen-affinity chromatography.
Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:1000.
beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX631830) diluted at a dilution of 1:1000.
beta Tubulin 3/ Tuj1 antibody [GT886] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:250.
beta III Tubulin antibody [GT886] detects beta III Tubulin proteins on embryonic mouse brain by immunohistochemical analysis.
Sample:Frozen section of embryonic mouse brain (mE18.5).
Green: beta III Tubulin antibody [GT886] (GTX631830) diluted at 1:2000.
Red: Sox2 antibody (GTX101506) diluted at 1:250.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:1000.
beta Tubulin 3/ Tuj1 antibody [GT886] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin 3/ Tuj1 protein stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Immunoprecipitation of beta III Tubulin protein from SK-N-SH whole cell extracts using 5 μg of beta III Tubulin antibody [GT886] (GTX631830).
Western blot analysis was performed using beta III Tubulin antibody [GT886] (GTX631830).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
beta Tubulin 3/ TUJ1 antibody [GT886] detects beta Tubulin 3/ TUJ1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT886] (GTX631830) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody (GTX129913) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).