Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Tubulin 3/ Tuj1. The exact sequence is proprietary.
Conjugation:
Unconjugated
Alternative Names:
tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4
Clonality:
Monoclonal
Concentration:
1 mg/ml (Please refer to the vial label for the specific concentration.)
Clone Designation:
[GT886]
Molecular Weight:
50
Isotype:
IgG1
Sensitivity:
beta III Tubulin antibody [GT886] specifically recognizes endogenous levels of beta III Tubulin based on the immunogen sequence.
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX631830 IHC-P Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:1000.
beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX631830) diluted at a dilution of 1:1000.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:1000.
Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Immunoprecipitation of beta III Tubulin protein from SK-N-SH whole cell extracts using 5 μg of beta III Tubulin antibody [GT886] (GTX631830). Western blot analysis was performed using beta III Tubulin antibody [GT886] (GTX631830). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
beta Tubulin 3/ TUJ1 antibody [GT886] detects beta Tubulin 3/ TUJ1 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT886] (GTX631830) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody (GTX129913) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
beta III Tubulin antibody [GT886] detects beta III Tubulin proteins on embryonic mouse brain by immunohistochemical analysis. Sample:Frozen section of embryonic mouse brain (mE18.5). Green: beta III Tubulin antibody [GT886] (GTX631830) diluted at 1:2000. Red: Sox2 antibody (GTX101506) diluted at 1:250.
beta Tubulin 3/ Tuj1 antibody [GT886] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Tubulin 3/ Tuj1 protein stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500. Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
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