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GTX635808 ICC/IF Image |
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SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] detects SARS-CoV-2 (COVID-19) nucleocapsid protein at cytoplasm by immunofluorescent analysis.Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) diluted at 1:2000. |
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Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as detection antibody at concentration of 1 μg/mL.Sensitivity : 39.06 pg/mL |
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Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) (8*103-9.5*10-3 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.EC50 : 82.34 nM |
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Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. |
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Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. |