SARS-CoV-2 (COVID-19) Nucleocapsid antibody [GT113], IgG2b, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) Nucleocapsid antibody [GT113], IgG2b, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: GTX635808
• Supplier Catalog Number: GTX635808
• Alternative Catalog Number: GTX635808-100,GTX635808-25
• Manufacturer: GeneTex
• Host: Mouse
• Category: Antikörper
• Application: ELISA, ICC, WB
• Species Reactivity: Virus
• Immunogen: Full length SARS-CoV-2 (COVID-19) nucleocapsid protein. (SARS-CoV-2 (strain Wuhan-Hu-1))
• Conjugation: Unconjugated
• Alternative Names: 2019-nCoV NP, 2019-nCoV Nucleocapsid, COVID-19 NP, COVID-19 Nucleocapsid, SARS-CoV-2 NP, SARS-CoV-2 Nucleocapsid, covid-19, sars-cov-2

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Clone Designation: [GT113]
• Isotype: IgG2b
• Sensitivity: This antibody detects SARS-CoV-2 nucleocapsid protein, but does not cross-react with SARS-CoV or MERS-CoV nucleocapsid proteins based on our internal testing.
• Buffer: PBS, no Preservative.
• Purity: Affinity purified by Protein A.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX635808 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] detects SARS-CoV-2 (COVID-19) nucleocapsid protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) diluted at 1:2000.

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as detection antibody at concentration of 1 μg/mL.
Sensitivity : 39.06 pg/mL

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) (8*10³-9.5*10^-3 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 82.34 nM

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.