SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745], IgG2a, Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745], IgG2a, Unconjugated, Mouse, Monoclonal
• Artikelnummer: GTX636037
• Hersteller Artikelnummer: GTX636037
• Alternativnummer: GTX636037-100,GTX636037-25
• Hersteller: GeneTex
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, IHC-P, WB
• Spezies Reaktivität: Virus
• Immunogen: Recombinant protein encompassing a sequence within the extracellular domain of SARS-CoV-2 (COVID-19) Spike S2. The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: covid-19, sars-cov-2

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Klon-Bezeichnung: [GT745]
• Isotyp: IgG2a
• Puffer: PBS, no Preservative.
• Reinheit: Affinity purified by Protein G.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:5000-1:1000000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX636037 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745] (GTX636037) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1039] (GTX635911) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745] (GTX636037) diluted at 1:1000000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745] (GTX636037) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745] detects SARS-CoV-2 (COVID-19) Spike S2 protein at cytoplasm by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745] (GTX636037) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min