Recombinant protein encompassing a sequence within the extracellular domain of SARS-CoV-2 (COVID-19) Spike S2. The exact sequence is proprietary.
Conjugation:
Unconjugated
Alternative Names:
covid-19, sars-cov-2
Clonality:
Monoclonal
Concentration:
1 mg/ml (Please refer to the vial label for the specific concentration.)
Clone Designation:
[GT745]
Isotype:
IgG2a
Buffer:
PBS, no Preservative.
Purity:
Affinity purified by Protein G.
Form:
Liquid
Application Notes:
WB: 1:5000-1:1000000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX636037 ELISA Image
Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745] (GTX636037) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1039] (GTX635911) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745] (GTX636037) diluted at 1:1000000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745] (GTX636037) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745] detects SARS-CoV-2 (COVID-19) Spike S2 protein at cytoplasm by immunohistochemical analysis.Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745] (GTX636037) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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