Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Asp19-Ser258
Target-Kategorie:
B7-H2/ICOSLG
Human ICOS, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200).
Immobilized Human B7-H2, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.2 ng/ml determined by ELISA (QC Test).
The purity of Human B7-H2 is greater than 90% as determined by SEC-HPLC.
Human B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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