Non-biotinylated Human B7-H2/ICOSLG Protein (BH7-HM472)

Product Overview

• Article Name: Non-biotinylated Human B7-H2/ICOSLG Protein (BH7-HM472)
• Biozol Catalog Number: KAB-BH7-HM472
• Supplier Catalog Number: BH7-HM472
• Alternative Catalog Number: KAB-BH7-HM472-100UG, KAB-BH7-HM472-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD275, B7H2, B7-H2, B7RP-1, GL50, ICOSL, ICOS-L, ICOSLG, B7RP1, LICOS, ICOS ligand, B7-H2B7-related protein 1, B7RP-1, B7RP1B7-like protein Gl50

Product Description

Recombinant Human B7-H2/ICOSLG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp19-Ser258.

Product Properties

• Molecular Weight: The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: O75144
• Buffer: Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
• Form: Lyophilized
• Sequence: Asp19-Ser258
• Target: B7-H2/ICOSLG

Images

Human ICOS, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200).

Immobilized Human B7-H2, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.2 ng/ml determined by ELISA (QC Test).

The purity of Human B7-H2 is greater than 90% as determined by SEC-HPLC.

Human B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.