Biotinylated Human Fc gamma RIIB/C (CD32b/c) Protein (CDB-HM401B)

Produktübersicht

• Artikelname: Biotinylated Human Fc gamma RIIB/C (CD32b/c) Protein (CDB-HM401B)
• Artikelnummer: KAB-CDB-HM401B
• Hersteller Artikelnummer: CDB-HM401B
• Alternativnummer: KAB-CDB-HM401B-100UG, KAB-CDB-HM401B-500UG
• Hersteller: KactusBio
• Kategorie: Proteine/Peptide
• Spezies Reaktivität: Human
• Konjugation: Biotin
• Alternative Synonym: Fc gamma RII, Fc gamma RIIB, Fc gamma RIIC, CD32, CD32b, CD32c, CDw32, FCG2, IGFR2, CD32A, FCG2 , FcGR, FCGR2, FCGR21, FCGR2A, FcgammaRIIB, Fc gammaRIIB, Fcgamma RIIB, Fc gamma RIIB

Produktbeschreibung

Recombinant Biotinylated Human Fc gamma RIIB/C (CD32b/c) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala46-Pro217.

Produkteigenschaften

• Molekulargewicht: The protein has a predicted MW of 22.5 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: P31994
• Puffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Reinheit: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Formulierung: Lyophilized
• Sequenz: Ala46-Pro217
• Target-Kategorie: Fc gamma RIIB/C (CD32b/c)

Bilder

The purity of Biotinylated Human Fc gamma RIIB/C is greater than 95% as determined by SEC-HPLC.

Biotinylated Human Fc gamma RIIB/C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIB/C, His-Avi Tag with an affinity constant of 3.97 uM as determined in SPR assay (Biacore T200).

Biotinylated Human Fc gamma RIIB/C, His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 5.87 uM as determined in SPR assay (Biacore T200).

Loaded Biotinylated Human Fc gamma RIIB/C, His-Avi Tag on Streptavidin-Biosensor can bind Rituximab with an affinity constant of 2.40 µM as determined in BLI assay (Gator Prime).