Biotinylated Human Fc gamma RIIB/C (CD32b/c) Protein (CDB-HM401B)
Biozol Catalog Number:
KAB-CDB-HM401B
Supplier Catalog Number:
CDB-HM401B
Alternative Catalog Number:
KAB-CDB-HM401B-100UG, KAB-CDB-HM401B-500UG
Manufacturer:
KactusBio
Category:
Proteine/Peptide
Species Reactivity:
Human
Conjugation:
Biotin
Alternative Names:
Fc gamma RII, Fc gamma RIIB, Fc gamma RIIC, CD32, CD32b, CD32c, CDw32, FCG2, IGFR2, CD32A, FCG2 , FcGR, FCGR2, FCGR21, FCGR2A, FcgammaRIIB, Fc gammaRIIB, Fcgamma RIIB, Fc gamma RIIB
Recombinant Biotinylated Human Fc gamma RIIB/C (CD32b/c) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala46-Pro217.
Molecular Weight:
The protein has a predicted MW of 22.5 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Ala46-Pro217
Target:
Fc gamma RIIB/C (CD32b/c)
The purity of Biotinylated Human Fc gamma RIIB/C is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Fc gamma RIIB/C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIB/C, His-Avi Tag with an affinity constant of 3.97 uM as determined in SPR assay (Biacore T200).
Biotinylated Human Fc gamma RIIB/C, His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 5.87 uM as determined in SPR assay (Biacore T200).
Loaded Biotinylated Human Fc gamma RIIB/C, His-Avi Tag on Streptavidin-Biosensor can bind Rituximab with an affinity constant of 2.40 µM as determined in BLI assay (Gator Prime).
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