Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Leu25-Ser645
Target-Kategorie:
EGFR/HER1
The purity of Human EGFR is greater than 95% as determined by SEC-HPLC.
Human EGFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human EGFR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA.
Loaded Human EGF, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.25 µM as determined in BLI assay (Gator Prime).
Loaded Human TGF-alpha, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.27 µM as determined in BLI assay (Gator Prime).
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