Human EGFR/HER1 Protein (EGF-HM101)

Product Overview

• Article Name: Human EGFR/HER1 Protein (EGF-HM101)
• Biozol Catalog Number: KAB-EGF-HM101
• Supplier Catalog Number: EGF-HM101
• Alternative Catalog Number: KAB-EGF-HM101-100UG, KAB-EGF-HM101-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: ErbB, EC 2.7.10, EC 2.7.10.1, EGFR, mENA, LEGFR, ERBB, ERBB1, HER1, PIG61, NISBD2

Product Description

Recombinant Human EGFR/HER1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu25-Ser645.

Product Properties

• Molecular Weight: The protein has a predicted MW of 70.19 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: P00533
• Buffer: Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Leu25-Ser645
• Target: EGFR/HER1

Images

The purity of Human EGFR is greater than 95% as determined by SEC-HPLC.

Human EGFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Immobilized Human EGFR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA.

Loaded Human EGF, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.25 µM as determined in BLI assay (Gator Prime).

Loaded Human TGF-alpha, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.27 µM as determined in BLI assay (Gator Prime).