Lyophilized from 0.22 µm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
E.coli
Reinheit:
> 95% as determined by Bis-Tris PAGE
Formulierung:
Lyophilized
Sequenz:
Ala197-Ile308
Target-Kategorie:
GDF15
Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).
Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8 ng/ml.
Human GDF15 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
Immobilized Human GDF15, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 13.1 ng/ml determined by ELISA (QC Test).
Immobilized Human GDF15, His Tag at 0.5ug/ml(100ul/well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA.
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