Human GDF15 Protein (GDF-HE115)

Product Overview

• Article Name: Human GDF15 Protein (GDF-HE115)
• Biozol Catalog Number: KAB-GDF-HE115
• Supplier Catalog Number: GDF-HE115
• Alternative Catalog Number: KAB-GDF-HE115-100UG, KAB-GDF-HE115-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: GDF-15, MIC-1, NAG-1, PDF, PLAB, PTGFB, GDF15, MIC1, RG-1, Placental TGF-beta, PTGF-beta, PTGFBPTGF-beta, Placental TGF-beta, PTGF-beta, PTGFBPTGF-beta

Product Description

Recombinant Human GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ala197-Ile308.

Product Properties

• Molecular Weight: The protein has a predicted MW of 13.5 kDa. The protein migrates to 15-16 kDa based on Bis-Tris PAGE result.
• Tag: N-His
• UniProt: Q99988
• Buffer: Lyophilized from 0.22 µm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: E.coli
• Purity: > 95% as determined by Bis-Tris PAGE
• Form: Lyophilized
• Sequence: Ala197-Ile308
• Target: GDF15

Images

Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).

Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8 ng/ml.

Human GDF15 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.

Immobilized Human GDF15, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 13.1 ng/ml determined by ELISA (QC Test).

Immobilized Human GDF15, His Tag at 0.5ug/ml(100ul/well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA.