Biotinylated Human IL-13 Protein (IL1-HM413B)

Produktübersicht

• Artikelname: Biotinylated Human IL-13 Protein (IL1-HM413B)
• Artikelnummer: KAB-IL1-HM413B
• Hersteller Artikelnummer: IL1-HM413B
• Alternativnummer: KAB-IL1-HM413B-100UG, KAB-IL1-HM413B-500UG
• Hersteller: KactusBio
• Kategorie: Proteine/Peptide
• Spezies Reaktivität: Human
• Konjugation: Biotin
• Alternative Synonym: IL13, IL-13, interleukin 13, NC30, P600, ALRH, BHR1, MGC116786, MGC116788, MGC116789

Produktbeschreibung

Recombinant Biotinylated Human IL-13 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gly21-Asn132.

Produkteigenschaften

• Molekulargewicht: The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 15 kDa and 35-45 kDa based on Bis-Tris PAGE result.
• Tag: N-His-Avi
• UniProt: AAK53823
• Puffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Reinheit: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Formulierung: Lyophilized
• Sequenz: Gly21-Asn132
• Target-Kategorie: IL-13

Bilder

Biotinylated IL-13 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of Biotinylated Human IL-13 is greater than 95% as determined by SEC-HPLC.

Human IL-13Ra1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human IL-13, His Tag with an affinity constant of 25.59 nM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human IL-13, His-Avi Tag at 5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test).

Loaded Biotinylated Human IL-13, His Tag on Anti-His-Biosensor can bind Human IL-13Ra1, hFc Tag with an affinity constant of 87.70 nM as determined in BLI assay (Gator Prime).