Recombinant Biotinylated Human IL-13 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gly21-Asn132.
Molecular Weight:
The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 15 kDa and 35-45 kDa based on Bis-Tris PAGE result.
Tag:
N-His-Avi
UniProt:
AAK53823
Buffer:
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Gly21-Asn132
Target:
IL-13
Biotinylated IL-13 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human IL-13 is greater than 95% as determined by SEC-HPLC.
Human IL-13Ra1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human IL-13, His Tag with an affinity constant of 25.59 nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human IL-13, His-Avi Tag at 5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test).
Loaded Biotinylated Human IL-13, His Tag on Anti-His-Biosensor can bind Human IL-13Ra1, hFc Tag with an affinity constant of 87.70 nM as determined in BLI assay (Gator Prime).
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