Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Met22-Pro141
Target-Kategorie:
TIGIT
Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 uM as determined in SPR assay (Biacore T200).
Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC.
Immobilized Human TIGIT, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 10.6ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA.
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