Human TIGIT Protein (TIG-HM210)

Product Overview

• Article Name: Human TIGIT Protein (TIG-HM210)
• Biozol Catalog Number: KAB-TIG-HM210
• Supplier Catalog Number: TIG-HM210
• Alternative Catalog Number: KAB-TIG-HM210-100UG, KAB-TIG-HM210-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: TIGIT, VSIG9, VSTM3, WUCAM

Product Description

Recombinant Human TIGIT Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Met22-Pro141.

Product Properties

• Molecular Weight: The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 48-50 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)
• UniProt: Q495A1
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Met22-Pro141
• Target: TIGIT

Images

Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 uM as determined in SPR assay (Biacore T200).

Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC.

Immobilized Human TIGIT, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 10.6ng/ml determined by ELISA (QC Test).

Immobilized Biotinylated Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA.