Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (30 ug of whole cell lysate) A: K562 10% SDS PAGE NCK1 antibody diluted at 1:
IHC of paraffin-embedded HeLa xenograft, using NCK1 antibody at 1:500 dilution.
Immunofluorescence of paraformaldehyde-fixed A431, using NCK1 antibody at 1:500 dilution.
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