NCK1 / NCK Antibody LS-C155317, Unconjugated, Polyclonal

Product Overview

• Article Name: NCK1 / NCK Antibody LS-C155317, Unconjugated, Polyclonal
• Biozol Catalog Number: LS-C155317-100
• Supplier Catalog Number: LS-C155317-100
• Alternative Catalog Number: LS-C155317-100
• Manufacturer: LifeSpan Biosciences
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IF, IHC, IHC-P, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NCK1. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: NCK1, Cytoplasmic protein nck, Cytoplasmic protein NCK1, NCK adaptor protein 1, Nck-1, NCKalpha, Melanoma NCK protein, NCK, NCK tyrosine kinase

Product Description

NCK antibody LS-C155317 is an unconjugated rabbit polyclonal antibody to human NCK (NCK1). Validated for ICC, IF, IHC and WB.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.78 mg/ml
• NCBI: 4690
• Buffer: 1X PBS, pH 7, 0.01% Thimerosal, 20% Glycerol, 1% BSA
• Purity: Immunoaffinity purified
• Form: 1X PBS, pH 7, 0.01% Thimerosal, 20% Glycerol, 1% BSA

Application Notes

• Application Dilute: ICC (1:100 - 1:1000), IF (1:100 - 1:1000), IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)
• Application Notes: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Images

Sample (30 ug of whole cell lysate) A: K562 10% SDS PAGE NCK1 antibody diluted at 1:

IHC of paraffin-embedded HeLa xenograft, using NCK1 antibody at 1:500 dilution.

Immunofluorescence of paraformaldehyde-fixed A431, using NCK1 antibody at 1:500 dilution.