Mouse anti Human CD14, conjugated with FITC, IgG2b, Clone: [FWKW], Monoclonal

Produktübersicht

• Artikelname: Mouse anti Human CD14, conjugated with FITC, IgG2b, Clone: [FWKW], Monoclonal
• Artikelnummer: NMB-0142
• Hersteller Artikelnummer: 0142
• Alternativnummer: NMB-0142
• Hersteller: NordicMubio
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC
• Spezies Reaktivität: Human
• Konjugation: FITC
• Alternative Synonym: CD14 FITC

Produktbeschreibung

Identification of human Monocyte/Macrophage cells expressing the 55 kDa M.W. surface antigen. CD14 is present on 70-93% of Monocyte/Macrophage cells in normal peripheral blood.

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: Titered for flow cytometry
• Klon-Bezeichnung: [FWKW]
• Isotyp: IgG2b
• UniProt: P08571
• Puffer: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
• Reinheit: Protein A/G Chromatography
• Formulierung: FITC
• Formel: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Anwendungsbeschreibung

• Anwendungsbeschreibung: PBMC: Add10 µl of MAB/10 6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.