Mouse anti Human CD14, conjugated with FITC, IgG2b, Clone: [FWKW], Monoclonal

Product Overview

• Article Name: Mouse anti Human CD14, conjugated with FITC, IgG2b, Clone: [FWKW], Monoclonal
• Biozol Catalog Number: NMB-0142
• Supplier Catalog Number: 0142
• Alternative Catalog Number: NMB-0142
• Manufacturer: NordicMubio
• Host: Mouse
• Category: Antikörper
• Application: FC
• Species Reactivity: Human
• Conjugation: FITC
• Alternative Names: CD14 FITC

Product Description

Identification of human Monocyte/Macrophage cells expressing the 55 kDa M.W. surface antigen. CD14 is present on 70-93% of Monocyte/Macrophage cells in normal peripheral blood.

Product Properties

• Clonality: Monoclonal
• Concentration: Titered for flow cytometry
• Clone Designation: [FWKW]
• Isotype: IgG2b
• UniProt: P08571
• Buffer: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
• Purity: Protein A/G Chromatography
• Form: FITC
• Formula: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Application Notes

• Application Notes: PBMC: Add10 µl of MAB/10 6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.