Sheep anti BrdU (bromodeoxyuridine), Unconjugated

Artikelnummer: NMB-A205P
Artikelname: Sheep anti BrdU (bromodeoxyuridine), Unconjugated
Artikelnummer: NMB-A205P
Hersteller Artikelnummer: A205P
Alternativnummer: NMB-A205P
Hersteller: NordicMubio
Wirt: Sheep
Kategorie: Antikörper
Applikation: FC, IHC, IP, WB
Konjugation: Unconjugated
Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections
Konzentration: See vial for concentration
Isotyp: IgG
Puffer: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Quelle: Sheep were immunized with bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin.
Reinheit: Ammonium Sulfate Precipitation
Formulierung: Unconjugated
Formel: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Anwendungsbeschreibung: Applications: Immunoprecipitation: 25-100 µg/ml, Immunohistochemistry: 25-100 µg/ml. The antibody has been tested using immunoprecipitation against 5-methyl cytosine (5-MeC) and bromodeoxyuridine (BrdU) or a control (no antigen). At a concentration of 25 ug/ml, this product demonstrates 8-fold higher reactivity with 5-MeC versus BrdU. For best results, use product at 25-100µg/ml. Note: Denature DNA sample first so that bases are accessible to anti BrdU antibody. Applicable in flow cytometry for the quantification of Brdu labeled cells, indication the proliferative activity of the tested cell population.