Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections
Concentration:
See vial for concentration
Isotype:
IgG
Buffer:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Source:
Sheep were immunized with bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin.
Purity:
Ammonium Sulfate Precipitation
Form:
Unconjugated
Formula:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Application Notes:
Applications: Immunoprecipitation: 25-100 µg/ml, Immunohistochemistry: 25-100 µg/ml. The antibody has been tested using immunoprecipitation against 5-methyl cytosine (5-MeC) and bromodeoxyuridine (BrdU) or a control (no antigen). At a concentration of 25 ug/ml, this product demonstrates 8-fold higher reactivity with 5-MeC versus BrdU. For best results, use product at 25-100µg/ml. Note: Denature DNA sample first so that bases are accessible to anti BrdU antibody. Applicable in flow cytometry for the quantification of Brdu labeled cells, indication the proliferative activity of the tested cell population.
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