Mouse anti Human CD2 APC, IgG2a, Clone: [T6.3], Monoclonal

Produktübersicht

• Artikelname: Mouse anti Human CD2 APC, IgG2a, Clone: [T6.3], Monoclonal
• Artikelnummer: NMB-AP02
• Hersteller Artikelnummer: AP02
• Alternativnummer: NMB-AP02
• Hersteller: NordicMubio
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC
• Spezies Reaktivität: Human
• Immunogen: Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T Lymphocytes activated by mixed lymphocyte culture.
• Konjugation: APC
• Alternative Synonym: T-cell surface antigen CD2, CD2 cytoplasmic domain-binding protein

Produktbeschreibung

Identification of human T cells and subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen.

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: Titered for flow cytometry
• Klon-Bezeichnung: [T6.3]
• Isotyp: IgG2a
• UniProt: P06729
• Puffer: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
• Reinheit: Protein A/G Chromatography
• Formulierung: APC
• Formel: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Anwendungsbeschreibung

• Anwendungsbeschreibung: PBMC: Add10 µl of MAB/10 6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. . See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.