Mouse anti Human CD2 APC, IgG2a, Clone: [T6.3], Monoclonal

Product Overview

• Article Name: Mouse anti Human CD2 APC, IgG2a, Clone: [T6.3], Monoclonal
• Biozol Catalog Number: NMB-AP02
• Supplier Catalog Number: AP02
• Alternative Catalog Number: NMB-AP02
• Manufacturer: NordicMubio
• Host: Mouse
• Category: Antikörper
• Application: FC
• Species Reactivity: Human
• Immunogen: Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T Lymphocytes activated by mixed lymphocyte culture.
• Conjugation: APC
• Alternative Names: T-cell surface antigen CD2, CD2 cytoplasmic domain-binding protein

Product Description

Identification of human T cells and subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen.

Product Properties

• Clonality: Monoclonal
• Concentration: Titered for flow cytometry
• Clone Designation: [T6.3]
• Isotype: IgG2a
• UniProt: P06729
• Buffer: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
• Purity: Protein A/G Chromatography
• Form: APC
• Formula: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Application Notes

• Application Notes: PBMC: Add10 µl of MAB/10 6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. . See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.