beta Catenin Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: beta Catenin Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-13-991
• Hersteller Artikelnummer: 13-991
• Alternativnummer: PRS-13-991-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IHC, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 650-750 of human beta Catenin (NP_001895.1).
• Konjugation: Unconjugated
• Alternative Synonym: CTNNB1, CTNNB, MRD19, armadillo

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 105kDa
• NCBI: 1499
• UniProt: P35222
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:200

Bilder

Western blot analysis of extracts of various cell lines, using beta Catenin antibody (13-991) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Western blot analysis of extracts from normal (control) and beta Catenin knockout (KO) 293T cells, using beta Catenin antibody (13-991) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat brain using beta Catenin antibody (13-991) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using beta Catenin antibody (13-991) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using beta Catenin antibody (13-991) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using beta Catenin antibody (13-991) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat fallopian tube using beta Catenin antibody (13-991) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using beta Catenin antibody (13-991) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using beta Catenin antibody (13-991) at dilution of 1:150 (40x lens).