beta Catenin Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: beta Catenin Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-13-991
• Supplier Catalog Number: 13-991
• Alternative Catalog Number: PRS-13-991-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 650-750 of human beta Catenin (NP_001895.1).
• Conjugation: Unconjugated
• Alternative Names: CTNNB1, CTNNB, MRD19, armadillo

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 105kDa
• NCBI: 1499
• UniProt: P35222
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using beta Catenin antibody (13-991) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Western blot analysis of extracts from normal (control) and beta Catenin knockout (KO) 293T cells, using beta Catenin antibody (13-991) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat brain using beta Catenin antibody (13-991) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using beta Catenin antibody (13-991) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using beta Catenin antibody (13-991) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using beta Catenin antibody (13-991) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat fallopian tube using beta Catenin antibody (13-991) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using beta Catenin antibody (13-991) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using beta Catenin antibody (13-991) at dilution of 1:150 (40x lens).