ACSL3 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-14-096
Artikelname: ACSL3 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-14-096
Hersteller Artikelnummer: 14-096
Alternativnummer: PRS-14-096-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 42-300 of human ACSL3 (NP_004448.2).
Konjugation: Unconjugated
Alternative Synonym: ACSL3, ACS3, FACL3, PRO2194
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 75kDa
NCBI: 2181
UniProt: O95573
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using ACSL3 antibody (14-096) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human mammary cancer using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).