ACSL3 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-14-096
Article Name: ACSL3 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-14-096
Supplier Catalog Number: 14-096
Alternative Catalog Number: PRS-14-096-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 42-300 of human ACSL3 (NP_004448.2).
Conjugation: Unconjugated
Alternative Names: ACSL3, ACS3, FACL3, PRO2194
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 75kDa
NCBI: 2181
UniProt: O95573
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using ACSL3 antibody (14-096) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human mammary cancer using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).