DDX3Y Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: DDX3Y Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-14-107
• Hersteller Artikelnummer: 14-107
• Alternativnummer: PRS-14-107-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DDX3Y (NP_001116137.1).
• Konjugation: Unconjugated
• Alternative Synonym: ATP-dependent RNA helicase DDX3Y, DEAD box protein 3, Y-chromosomal, DDX3Y, DBY

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 73kDa
• NCBI: 8653
• UniProt: O15523
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Bilder

Western blot analysis of extracts of various cell lines, using DDX3Y antibody (14-107) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat testis using DDX3Y antibody (14-107) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using DDX3Y antibody (14-107) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using DDX3Y antibody (14-107) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using DDX3Y antibody (14-107) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using DDX3Y Polyclonal Antibody (14-107) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using DDX3Y Polyclonal Antibody (14-107) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using DDX3Y Polyclonal Antibody (14-107) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.