DDX3Y Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: DDX3Y Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-14-107
• Supplier Catalog Number: 14-107
• Alternative Catalog Number: PRS-14-107-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DDX3Y (NP_001116137.1).
• Conjugation: Unconjugated
• Alternative Names: ATP-dependent RNA helicase DDX3Y, DEAD box protein 3, Y-chromosomal, DDX3Y, DBY

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 73kDa
• NCBI: 8653
• UniProt: O15523
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Images

Western blot analysis of extracts of various cell lines, using DDX3Y antibody (14-107) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat testis using DDX3Y antibody (14-107) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using DDX3Y antibody (14-107) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using DDX3Y antibody (14-107) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using DDX3Y antibody (14-107) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using DDX3Y Polyclonal Antibody (14-107) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using DDX3Y Polyclonal Antibody (14-107) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using DDX3Y Polyclonal Antibody (14-107) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.