RPN1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-14-462
Artikelname: RPN1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-14-462
Hersteller Artikelnummer: 14-462
Alternativnummer: PRS-14-462-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human RPN1 (NP_002941.1).
Konjugation: Unconjugated
Alternative Synonym: RPN1, DKFZp686B16177, OST1, RBPH1
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 68kDa
NCBI: 6184
UniProt: P04843
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using RPN1 antibody (14-462) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using RPN1 antibody (14-462) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).