RPN1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-14-462
Article Name: RPN1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-14-462
Supplier Catalog Number: 14-462
Alternative Catalog Number: PRS-14-462-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human RPN1 (NP_002941.1).
Conjugation: Unconjugated
Alternative Names: RPN1, DKFZp686B16177, OST1, RBPH1
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 68kDa
NCBI: 6184
UniProt: P04843
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using RPN1 antibody (14-462) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using RPN1 antibody (14-462) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).