CTCF Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: CTCF Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-14-881
• Hersteller Artikelnummer: 14-881
• Alternativnummer: PRS-14-881-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ChIP, IF, IHC, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).
• Konjugation: Unconjugated
• Alternative Synonym: CTCF, MRD21

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 140kDa
• NCBI: 10664
• UniProt: P49711
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:1000 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100ChIP: 1:50 - 1:200

Bilder

Western blot analysis of extracts of 293T cells, using CTCF antibody (14-881) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Immunohistochemistry of paraffin-embedded rat liver using CTCF antibody (14-881) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using CTCF antibody (14-881) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat spleen using CTCF antibody (14-881) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using CTCF antibody (14-881) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of U2OS cells using CTCF antibody (14-881) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CTCF antibody (14-881). Western blot was performed from the immunoprecipitate using CTCF antibody (14-881) at a dilition of 1:1000.

Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using CTCF antibody (14-881) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.