CTCF Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: CTCF Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-14-881
• Supplier Catalog Number: 14-881
• Alternative Catalog Number: PRS-14-881-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).
• Conjugation: Unconjugated
• Alternative Names: CTCF, MRD21

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 140kDa
• NCBI: 10664
• UniProt: P49711
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:1000 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100ChIP: 1:50 - 1:200

Images

Western blot analysis of extracts of 293T cells, using CTCF antibody (14-881) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Immunohistochemistry of paraffin-embedded rat liver using CTCF antibody (14-881) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using CTCF antibody (14-881) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat spleen using CTCF antibody (14-881) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using CTCF antibody (14-881) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of U2OS cells using CTCF antibody (14-881) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CTCF antibody (14-881). Western blot was performed from the immunoprecipitate using CTCF antibody (14-881) at a dilition of 1:1000.

Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using CTCF antibody (14-881) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.