TEAD1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: TEAD1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-14-950
• Hersteller Artikelnummer: 14-950
• Alternativnummer: PRS-14-950-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ChIP, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 135-215 of human TEAD1 (NP_068780.2).
• Konjugation: Unconjugated
• Alternative Synonym: TEAD1, AA, REF1, TCF13, TEF-1, NTEF-1, TCF-13, TEAD-1

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 48kDa
• NCBI: 7003
• UniProt: P28347
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200CHIP: 1:20 - 1:50

Bilder

Western blot analysis of extracts of various cell lines, using TEAD1 antibody (14-950) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 120s.

Immunohistochemistry of paraffin-embedded human lung cancer using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophageal cancer using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF7 cells using TEAD1 antibody (14-950).

Chromatin immunoprecipitation analysis of extracts of HeLa cell line, using TEAD1 rabbit polyclonal antibody (14-950) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.