TEAD1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: TEAD1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-14-950
• Supplier Catalog Number: 14-950
• Alternative Catalog Number: PRS-14-950-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 135-215 of human TEAD1 (NP_068780.2).
• Conjugation: Unconjugated
• Alternative Names: TEAD1, AA, REF1, TCF13, TEF-1, NTEF-1, TCF-13, TEAD-1

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 48kDa
• NCBI: 7003
• UniProt: P28347
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200CHIP: 1:20 - 1:50

Images

Western blot analysis of extracts of various cell lines, using TEAD1 antibody (14-950) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 120s.

Immunohistochemistry of paraffin-embedded human lung cancer using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophageal cancer using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF7 cells using TEAD1 antibody (14-950).

Chromatin immunoprecipitation analysis of extracts of HeLa cell line, using TEAD1 rabbit polyclonal antibody (14-950) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.