Cytochrome c Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Cytochrome c Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-14-986
• Hersteller Artikelnummer: 14-986
• Alternativnummer: PRS-14-986-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human Cytochrome c (NP_061820.1).
• Konjugation: Unconjugated
• Alternative Synonym: CYCS, CYC, HCS, THC4, Cytochrome c, Cytochrome c, somatic

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 14kDa
• NCBI: 54205
• UniProt: P99999
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Bilder

Western blot analysis of extracts of various cell lines, using Cytochrome c antibody (14-986) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat liver using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).

Immunofluorescence analysis of HeLa cells using Cytochrome c antibody (14-986) at dilution of 1:100. Blue: DAPI for nuclear staining.