Cytochrome c Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Cytochrome c Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-14-986
• Supplier Catalog Number: 14-986
• Alternative Catalog Number: PRS-14-986-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human Cytochrome c (NP_061820.1).
• Conjugation: Unconjugated
• Alternative Names: CYCS, CYC, HCS, THC4, Cytochrome c, Cytochrome c, somatic

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 14kDa
• NCBI: 54205
• UniProt: P99999
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using Cytochrome c antibody (14-986) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat liver using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).

Immunofluorescence analysis of HeLa cells using Cytochrome c antibody (14-986) at dilution of 1:100. Blue: DAPI for nuclear staining.