FBL Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: FBL Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-15-018
• Hersteller Artikelnummer: 15-018
• Alternativnummer: PRS-15-018-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, IP, WB
• Spezies Reaktivität: Human, Monkey, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-321 of human FBL (NP_001427.2).
• Konjugation: Unconjugated
• Alternative Synonym: FBL, FIB, FLRN, RNU3IP1

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 37kDa
• NCBI: 2091
• UniProt: P22087
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50

Bilder

Western blot analysis of extracts of various cell lines, using FBL antibody (15-018) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded mouse liver using FBL antibody (15-018) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using FBL antibody (15-018) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using FBL antibody (15-018) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using FBL antibody (15-018) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using FBL antibody (15-018) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-251MG cells using FBL antibody (15-018) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug FBL antibody (15-018). Western blot was performed from the immunoprecipitate using FBL antibody (15-018) at a dilition of 1:1000.